Andrea Bagnacani, Markus Wolfien (RBC / de.STAIR)

04.09.2018. Full day course

Barbarastraße 21 (SL-Gebäude), 49076 Osnabrück, Germany

In this workshop, we provide a short summary of the current use and possible future applications of Next Generation Sequencing (NGS) technology in the clinical context. This will be followed by basic RNA-Seq data analysis procedures and a showcase project, which will demonstrate the data analysis of a clinical NGS dataset with the help of our recently published analysis workflow named TRAPLINE. Leveraging on TRAPLINE and the Galaxy environment, we guide the participants through all relevant steps for computational data analysis, as well as provide further suggestions to link the obtained results with current gene-disease related databases, or other relevant resources to promote the clinical impact of the technology. Finally, we will discuss our attendees’ NGS experiments, and provide advice on their NGS data analysis approaches.

Learning goals:
(I) get familiar with the Galaxy framework ( for carrying out scientific data analyses, (ii) learn methods for data exploration and quality control of NGS datasets, (iii) get familiar with the concepts underlying genome alignment, and data visualization of subsequent results.

This workshop is intended for MSc/PhD students, postdocs and researchers with background in life science research.

Galaxy, Reproducibility, Workflow development, RNA-Seq data analysis, Visualization.


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