Jacobo Miranda Ackermann, Fadi al Machot, Nils Hoffmann (BioInfra.Prot / LIFS)
November 11th, 2018
ISAS Campus, Dortmund, Germany (as part of Lipidomics Forum)
In this course, we will work through a typical use-case for bioinformatics processing of shotgun lipidomics data following MS acquisition, starting with PeakStrainer and LipidXplorer for filtering, identification and quantification of lipid signals, followed by a comparison of the lipidomes using the LUX Score lipidome homology, before finally checking our quantitative lipidome results against those stored in LipidCompass. The course will consist of a short theory and background overview of the employed programs complemented by the application of the tools to a provided data set. Participants are required to bring their own laptop in order to work on the hands-on exercises.
Participants will be able to understand and explain the shotgun workflow. They will learn the fundamentals of the software tools used and how to choose parameters for them. They will learn to understand and interpret the results of each step of the pipeline.
Basic knowledge of lipidomics, analytical workflows in lipidomics and basic familiarity with web-based applications. Participants are required to bring their own laptop in order to work on the hands-on exercises.
Lipidomics, Shotgun, Pipeline
PeakStrainer and LipidXplorer, LUX Score and LipidCompass.