SABIO-RK (http://sabiork.h-its.org/) is a manually curated database containing structured information about biochemical reactions and their corresponding kinetics mainly extracted from scientific literature. It describes participants and modifiers of the reactions, as well as measured kinetic data embedded in their experimental and environmental context. The system offers standardized data for metabolic, transport and signalling reactions from all kinds of organisms. It enables the access to and export of kinetic data for experimentalists and modellers. Thereby, SABIO-RK facilitates the exchange of the data between experimentalists and modellers, supporting the setup of quantitative computer models. The data in SABIO-RK is either manually extracted from published literature or directly submitted by experimenters. It is curated by experts and supplemented with additional information from other databases and biological ontologies. The database can be accessed either manually via a web-based-search interface or programmatically via REST-ful web services that also allow direct automated access by other tools. Both interfaces support the export of the data in different formats. Confidential data, such as directly submitted pre-publication data, can be hidden for public access by defining the sharing rights for named users.