Tracy
Tracy is a command-line tool for the analysis of Sanger chromatogram trace files. It supports basecalling, alignment, assembly, and deconvolution of Sanger sequencing data, enabling robust processing of common capillary sequencing outputs. As part of the GEAR toolbox, Tracy can be used through genome analysis workflows and integrated into automated or reproducible pipelines.
Key benefits
Processes Sanger chromatogram trace files
Supports basecalling and sequence alignment
Enables assembly of Sanger sequencing reads
Provides deconvolution functionality for mixed trace signals
Suitable for integration into command-line and pipeline-based workflows
Applications
Analysis of Sanger sequencing traces
Basecalling from chromatogram files
Alignment of Sanger reads to reference sequences
Assembly of sequencing reads from capillary sequencing experiments
Deconvolution of mixed or overlapping chromatogram signals
Intended use
Tracy is intended for molecular biologists, geneticists, sequencing facilities, and bioinformaticians working with Sanger sequencing data. It is particularly suited for users who need reproducible command-line processing of chromatogram trace files, either as a standalone tool or as part of broader genome analysis workflows.
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