HyPPI
HyPPI is a web-based tool for classifying protein–protein complexes as permanent, transient, or crystal artifacts. Permanent complexes are stable only in their associated state, whereas transient complexes can remain stable both as complexes and as individual subunits. Crystal artifacts are non-biological contacts formed during crystallization.
HyPPI performs the classification using two structural properties of the protein–protein interface: its hydrophobicity (ΔG hydrophobic) and the interface quotient (IF-quotient), which reflects the symmetry of the interacting surfaces.
Key benefits
Automated classification of protein–protein complexes
Distinguishes permanent and transient interactions from crystal artifacts
Uses interpretable structural interface descriptors
Supports assessment of the biological relevance of crystallographic contacts
Web-based analysis without local software installation
Applications
Classification of protein–protein interactions in crystal structures
Identification of potentially biologically relevant protein complexes
Detection of non-functional crystallization contacts
Analysis of interface hydrophobicity and symmetry
Support for structural interpretation and protein interaction studies
Intended use
HyPPI is intended for structural biologists, protein scientists, bioinformaticians, and computational biologists working with three-dimensional protein complex structures. It is particularly useful for researchers who need to assess whether an observed protein–protein interface represents a permanent or transient biological interaction or is likely to be a crystal artifact.
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