A training tutorial hosted jointly by the service centers ‘Bioinformatics for Proteomics’ (BioInfra.Prot) and the ‘Center for Integrative Bioinformatics’ (CIBI) at the German Conference on Bioinformatics (GCB) 2015.
BioInfra.Prot: Martin Eisenacher, Michael Kohl, Michael Turewicz, Julian Uszkoreit
CIBI: Julianus Pfeuffer, Timo Sachsenberg
The ease of use of bioinformatics software and workflows for the analysis of high-throughput proteomics experiments blurs the complexity of this analysis and the interdependence of the consecutive working steps applied. This tutorial will ‘open the black box’: the most important parts of a proteomics workflows and proteomics software will be reviewed regarding their relationships, pitfalls and consequences of crucial decisions. Supervised by experts in the field, the participants will be given theoretical basics and will then be able to explore real-world datasets on their own. The tutorial covers all basic topics of the analysis of large-scale proteomics data, starting from fundamentals of the data generation to the fully automated processing of large data sets through workflow systems. Topics covered are Sequence databases, Spectra identification and Decoy approach, Protein inference, Protein quantification and Workflow systems (OpenMS/KNIME) for data processing.
The participants learn about the basics of data analysis in the proteomics area. They will gain an improved understanding of the possibilities and limitations of standard approaches and bioinformatics tools in the field, which are often used in a rather unreflected manner.
The tutorial is aimed at Master and PhD students, postdoctoral fellows or other scientists with basic and advanced knowledge in proteomics or bioinformatics experts that start with MS analysis.
Bioinformatics for proteomics, Sequence databases, Spectra identification / decoy approach, Protein inference, Protein quantification, Workflow systems for data processing
DecoyDatabaseBuilder, PIA, ProLiC, KNIME, OpenMS
PD Dr. Martin Eisenacher
For more information see http://gcb2015.cs.tu-dortmund.de/tutorial1.php