Educators:
Frank Oliver Glöckner, Pelin Yilmaz, Jörg Peplies, Antonio Fernandez-Guerra, Jan Gerken, Ivaylo Kostadinov, Christian Quast (BioData)
Date:
22-11-2016 to 25-11-2016, 9:00-17:00
Location:
Max Planck Institute for Marine Microbiology Bremen
Celsiusstrasse 1
28359 Bremen
(lecture hall/PC-lab)
Contents:
Microbial Diversity analysis, using Next-Generation Sequencing technologies (NGS), have become a routine approach within the last years. Analysis of the ribosomal RNA gene (rDNA) represents the gold standard for microbial identification, phylogeny, and biodiversity studies. Sequencing efforts using this molecule as a marker have been so extensive, that the number of entries for this gene in public repositories has already exceeded 5 million. The understanding of the principles of marker-gene-based sequence analysis using NGS technologies is therefore essential for microbiology, biotechnology, quality control and even clinical diagnostics.
This training course explains all necessary steps researchers need to take when performing amplicon-based investigations using the rDNA as a marker gene. The course starts with experimental design, including an overview of relevant sequencing technologies, and the selection, design, and evaluation of primers for the amplification of rDNA. It teaches the importance of contextual data and standards for data analysis and integration. It provides examples and best practice solutions for data pre-processing and quality assurance up to the contextualized submission of the NGS data to public repositories. It finishes with a framework for statistical analysis of the data including a short LINUX and R crash course.
Learning goals:
- Appropriate experimental design
- Importance of contextual data and standards
- Quality control and quality assurance
- Data submission to public repositories
- Multivariate statistical techniques in microbial ecology
Prerequisites:
- The training course is targeted towards biologists that want to apply NGS technologies in their research.
- No programming skills are necessary.
- No prior knowledge about the tools is required.
Keywords:
Sequencing technologies, Primers, Contextual data, Standards, Quality assurance, Data submission, Biodiversity statistics
Tools:
ARB/SILVA/SILVAngs, Mothur, GUSTAME, MED
Contact:
More Information and Registration:
Please register until September 30th, 2016 at: http://www.ribocon.com/training_upcoming.html