Timo Sachsenberg, Julianus Pfeuffer (CIBI)
18 September 2017 14:00-17:00
Morgenstelle, University of Tuebingen
specific room will be announced in time, depending on number of registrations.
The course introduces key concepts of non-targeted label-free proteomics. Non-targeted methods are ideal for unbiased discovery studies and scale well for large-scale studies (e.g., clinical proteomics). Based on example datasets we will then introduce several open-source software tools for proteomics primarily focusing on OpenMS (www.openms.org). We will demonstrate how these tools can be combined into complex data analysis workflows including visualization of results. Participants will have the opportunity to design custom analysis workflows together with instructors.
Target audience are computational scientists interested in working with raw mass spectrometric data.
The participants should bring their own laptop computers. Installer versions of all required software will be available.
Learning goals: (3 hours)
- Introduction to computational mass spectrometry proteomics
- OpenMS and the integration platform KNIME
- Hands-on: Identification and Quantification workflow for Label-free quantitative proteomics
LC-MS-based proteomics and metabolomics, OpenMS, workflows, KNIME, data analysis