Julian Uszkoreit (BioInfra.Prot), Michael Turewicz (BioInfra.Prot) and Michael Kohl (BioInfra.Prot)

7. December 2017

Ruhr-University Bochum, Germany

In this one day course we will show some of our tools provided in de.NBI, namely “PIA – Protein Inference Algorithms”, “PAA – Protein Array Analyzer” and CalibraCurve. PIA allows to inspect and combine the results of proteomics search engines. The main focus lays on the integrated inference algorithms for identification and quantification purposes. PAA is an R/Bioconductor software package providing a comprehensive workflow for protein microarray data analysis, especially aimed at biomarker discovery. CalibraCurve is a tool intended for the generation of calibration curves in the context of MRM (targeted proteomics) experiments. Such calibration curves are necessary for the selection of suitable transitions.

Learning goals:
Attendees of the course will learn how to use the tools PIA, PAA and CalibraCurve effectively for their daily proteomics tasks.

This course is for all researches in the field of proteomics. The attendees should have basic knowledge of LC-MS proteomics, but no prior bioinformatics skills are required. Basic knowledge of how to analyse LC-MS data are sufficient. Attendees are required to bring their own laptops. If not possible, please contact the organizers.

Proteomics; Data Analysis;

PIA; PAA; CalibraCurve;

Julian Uszkoreit
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