• Kiel

 

Educators:
Marc Höppner (Associated partner - CAU)

Date:
September 19, 2019 - 08:30 - 16:30

Location:
Kiel, Am Botanischen Garten 1-9, Room E49

Contents:
The constant drop in sequencing prices and the development of easy-to-use assembly programs makes it possible, even for small groups, to embark on a de novo genome project to obtain the full sequence of their species of interest. However, automatically annotating all genetic features in a eukaryotic genome, especially in non-model species with few or no sequenced closely related species, remains a challenge and standard pipelines still do not exist.

Learning goals:
In this one day course participants will learn about these challenges and the current strategies that can be used to try to obtain the most complete set of genes from a de novo assembled eukaryotic genome. We will also discuss how additional data, such as RNA-seq, assembled transcriptomes or proteomic data, can be used to improve the annotation, which can inform decisions on how best to spend the budget during a genome project. The practical part of the course will explain how to automatically and manually annotate a genomic region and how that information can be immediately translated into biologically relevant data for the species of interest.

Prerequisites:
None

Keywords:
eukaryotic genome, annotation, RNAseq proteomic data, assembly

Contact:
Marc Höppner, This email address is being protected from spambots. You need JavaScript enabled to view it.

This course is designed for 15 participants. If we receive (a lot) more applications, we will choose participants based on the relevance of their application and/or a first-come-first-serve basis.

Registration:
https://forms.gle/ZkQ2RLwRJ9CXVmYH8

Registration deadline is 15th of August.