EBImage
EBImage is an R/Bioconductor package that provides a comprehensive set of tools for reading, writing, processing, and analyzing images. In the context of high-throughput microscopy-based cellular assays, EBImage offers specialized functionality to support image analysis and quantitative measurements.
Key Features
- General Purpose Image Processing: EBImage provides a wide range of functions for general purpose image processing, including image reading and writing, transformations, filtering, and thresholding.
- Cellular Assay Analysis: In the context of high-throughput microscopy-based cellular assays, EBImage offers specialized tools to:
- Transform images: apply various transformations to correct or enhance image features.
- Segment cells: identify and separate individual cells within an image.
- Extract quantitative cellular descriptors: measure various aspects of cell morphology, such as size, shape, and fluorescence intensity.
Use Cases
- High-Throughput Microscopy Analysis: Use EBImage to analyze large collections of microscopy images, extracting quantitative measurements of cellular features and phenotypes.
- Cell Segmentation and Quantification: Apply EBImage's segmentation tools to identify individual cells within an image, followed by measurement of cell size, shape, or other morphological features.
- Fluorescence Microscopy Analysis: Utilize EBImage's capabilities for fluorescence microscopy analysis, including image correction, thresholding, and feature extraction.
Availability
EBImage is available as an R/Bioconductor package and can be downloaded from the Bioconductor website. Also find this service in BioTools here.
Part of the Human Omics Analysis Toolbox.
Website