SANS is a freely available command line tool for phylogenetic reconstruction. It is alignment- and reference-free, and accepts (multiple) FASTA or FASTQ files containing complete genomes, assembled contigs, or raw reads as input. Sequence segments (in terms of k-mers) that are shared by a set of genomes are used as indicators for phylogenetic splits. Splits are combined, weighted, filtered and output in tab separated format. They can visualized as a phylogenetic tree or network using existing tools such as SplitsTree. SANS is implemented in C++, and converter scripts for newick and nexus are provided.


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Service provision and maintenance funded by de.NBI.