DEXSeq

DEXSeq is an extension of DESeq for the analysis of alternative exon usage, allowing researchers to identify differential exon usage between conditions or time points.

Key Features
  • Alternative Exon Usage Analysis: DEXSeq provides a comprehensive toolset for analyzing alternative exon usage from high-throughput sequencing data.
  • Extension of DESeq: DEXSeq builds upon the popular DESeq package, which is widely used for differential expression analysis of high-throughput sequencing assays.

Methodology: DEXSeq uses a similar approach to DESeq2, but instead of modeling gene-level counts, it models exon-level counts using the Gamma-Poisson distribution. This allows researchers to identify exons that are differentially used between conditions or time points.

Use Cases
  • Alternative Splicing Analysis: Use DEXSeq to analyze alternative splicing events from RNA-seq data and identify exons that are differentially included or excluded between conditions.
  • Differential Exon Usage Analysis: Apply DEXSeq to identify differential exon usage between conditions or time points, providing insights into the regulation of gene expression.
Availability

DEXSeq is available as an R/Bioconductor package and can be downloaded from the Bioconductor website.

 

Find this service in BioTools here.

Part of the Human Omics Analysis Toolbox.

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Funding: Neither development nor maintenance funded by de.NBI